On farm production of Mycorrhizae

Yanglem Herojit Singh, K Mamocha Singh, Kennedy Ninthoujam, Ng Taibangnganbi Chanu
Contd from previous issue
· Care should be taken during plants grow
Benefited Crops
Mycorrhiza or VAM suitable for all cereals, legumes, fruits and horticultural crops
Benefits of Mycorrhiza
· Produce more vigorous and healthy plants. Increase plant establishment and survival at seedling or transplanting.
· Enhance flowering and fruiting.
· Increase yields and crop quality.
· Improve drought tolerance, allowing watering reduction
· Optimize fertilizers use, especially phosphorous
· Increase tolerance to soil salinity.
· Reduce disease occurrence.
· Contribute to maintain soil quality ad nutrient cycling
· Contribute to control soil erosion.
Application of VAM fungi
Nursery application
· 100 g bulk inoculum is sufficient for one m2
· The inoculum should be applied a 2-3 cm below the soil at the time of sowing.
· The seeds/cuttings should be sown/planted above the VAM inoculum to cause infection.
For polythene bag raised crops
· 5 to 10 bulk inoculums is sufficient for each packet.
· Mix 1 kg of inoculum with 100 kg of potting mixture and pack the potting mixture in polythene bag before sowing.
For out-planting
· 20 g of VAM inoculum is required per seedling
· Apply inoculum at the time of planting.
For existing trees
· 200 g of VAM inoculum is required for inoculating one tree. Apply inoculum near the root surface at the time of fertilizer application
Application Dose: 3-4 kg acre-1
Economics of AMF Production
· In this on-farm production technique, the left over rhizospheric soil (after harvesting of roots) contains fairly good number of infectious propagules (more than 10 IP g-1 soil) and can be used to continue subsequent production cycles, to produce 10 lakhs propagules of AM fungi during every cropping season.
· The average cost of production is Rs. 400.00 during each production cycle for producing sufficient inoculum for application in one ha.
Assessment of Root Colonization
· Wash the surface feeder roots and cut the roots up to 1 cm.
· Heat the roots at 700C for 10-15 min (depend upon the age and tenderness of the roots) with 10% potassium hydroxide (KOH) solution.
· Pour off the KOH solution and rinse the roots thoroughly with water.
· Acidify the roots by immersing in 5% HCl for 5 min.
· Pour out the HCI and add staining solution (0.05% lactophenol tryphan blue) and keep them overnight for staining or boil for 10-15 min at 700C.
· Destain the roots with destaining solution (50% glycerol).
· Mount the root pieces on the glass slides and observe under microscope for mycorrhizal colonization.
Micorrhizal colonization is expressed using the following formula: Percentage of Colonization = Number of root segments with Mycorrhiza x 100.